Enzyme immunoassay of human urinary kallikrein
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چکیده
منابع مشابه
Enzyme Immunoassay of Human Urinary Kallikrein
An enzyme immunoassay for the determination of human urinary kallikrein has been developed and is compared with other human urinary kallikrein assays such äs radioimmunoassay, dog blood pressure assay, rat uterus test after kinin liberation and synthetic Substrate) tests (AcPheArgOEt and S-2266). The usable ränge of the Standard curve is from 0.05 to 12 ng kallikrein per test. The intfaassay co...
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Human urinary kallikrein (urokallikrein) has been purified by affinity chromatography with aprotinin coupled to CH-Sepharose and by gel filtration. The isolation procedure, which was performed under mild conditions, was completed in a 36-hr period and yielded an overall recovery of more than 75% and a purification of 1727-fold. Homogeneity of the urokallikrein was demonstrated by three criteria...
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The h i s t o r y of k a l l i k r e i n begins w i t h the d i s c o v e r y of u r i n a r y k a l l i k r e i n more than f i f t y years ago. The r e n a l o r i g i n of u r i n a r y k a l l i k r e i n , though not f i n a l l y proved, i s l a r g e l y accepted. A p o s s i b l e r o l e of k a l l i k r e i n i n the r e g u l a t i o n of kidney f u n c t i o n and blood pressure has...
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ژورنال
عنوان ژورنال: Fresenius' Zeitschrift für analytische Chemie
سال: 1982
ISSN: 0016-1152
DOI: 10.1007/bf00481760